Tmem192-rfp-3xha

Author: phoj

August undefined, 2024

WebSep 16, 2024 · A CAG-loxP-neomycin-Stop-loxP-FRT-TMEM192-3xHA-FRT-pA cassette incorporating full-length human TMEM192 cDNA coding sequences fused to a 3x … WebMedicaid Managed Care Requests for Applications. Please note: the official site for NCDHHS procurements is the North Carolina Interactive Purchasing System. …

TMEM192 transmembrane protein 192 [ (human)]

WebImmunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Save time and replace your secondary antibodies with our FlexAble Antibody Labeling Kits. WebAug 2, 2024 · Lysosomes promote cellular homeostasis through macromolecular hydrolysis within their lumen and metabolic signaling by the mTORC1 kinase on their limiting membranes. Both hydrolytic and signaling... otter out of water

TMEM219 Gene - GeneCards TM219 Protein TM219 Antibody

WebAgencies submitting a proposal in response to this RFP agree that the materials and submittals are prepared at the agency ’s own expense with the express understanding … WebAll lanes : Anti-TMEM192 antibody [EPR14330-67] (ab186737) at 1/10000 dilution Lane 1 : U87-MG cell lysate Lane 2 : Human fetal liver lysate Lane 3 : Human fetal lung lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution Predicted band size: 31 kDa Observed band size: … WebThe original EGFP was removed, and Tmem192-mRFP-3xHA was inserted at NheI and EcoRI sites into the pLJM1 backbone. Vector type Mammalian Expression, Lentiviral Selectable … otter park cornwall

pLJM1-Tmem192-mRFP-3xHA Addgene 134631 product …

WebMar 29, 2024 · TMEM192 transmembrane protein 192 [ (human)] Gene ID: 202431, updated on 29-Mar-2024. Summary. Enables protein homodimerization activity. Located in several … WebFeb 2, 2024 · A synthetic cDNA encoding TMEM192-RFP-3xHA was cloned into the pLJM1 lentiviral vector (Lim et al., 2024). TMEM192-FLAG was cloned also cloned in the pLJM1 vector by amplifying the TMEM192 cDNA using primers to append a DYKDDDK (“FLAG”) peptide to the c-terminus of the resulting protein. otter party bagsWeb(A) Localization of Tmem192-3xHAfusionproteintolysosomes.Tmem192-3xHAandlysosomeswere detected by immunofluorescence with a ntibodies to the HA … otterpac 25l waterproof foldable backpack

"Webprk5-TMEM192-3xHA PM mutant. Plasmid #149506. Purpose. Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA. Depositor. David Sabatini. Article. Adelmann et al Nature. 2024 Nov 18. pii: 10.1038/s41586. Insert. TMEM192 (TMEM192 Human) Use. Transient expression " - Tmem192-rfp-3xha

Tmem192-rfp-3xha

METABOLISM Lysosomal metabolomics reveals V-ATPase

WebJul 19, 2024 · HA-TMEM192-RFP construct allowed us to visualize the lysosomes (white), many of which contained PFF tagged with Alexa Fluor 488 (red). Scale bars, 5 μm for low magnification and 2.5 μm for the inset. (F) Confocal samples of U2OS cells described in (A) imaged using STED microscopy. Scale bars, 10 μm for low magnification, and 0.5 μm for … WebTMEM192-3xHA (HA) served as a loading control. (Right) Densitometric analysis of NPC1 level normalized to TMEM192-3xHA (HA). Data are presented as the mean + s.d. of 3 independent experiments. (I) (Left) Confocal analysis of Proximity Ligation Assay (PLA) between TMEM192-3xHA and NPC1. HA-lyso GSC#9 were treated as described in (H).

Did you know?

WebApr 22, 2024 · LysoTag TMEM192-mRFP-3xHA (TMRHA) was generated by subcloning the cDNA of TMEM192 (Origene) together with monomeric red fluorescent protein (mRFP) and 3× haemagglutinin (HA) tag into the NheI and ... WebImmunofluorescence analysis of HepG2 cells labeling TMEM192 with ab185545 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi. Western blot - Anti …

WebTMEM192 ( TMEM192 Human) Use Lentiviral Tags 3XHA Expression Mammalian Mutation Promoter Ubiquitin Availability Academic Institutions and Nonprofits only Enlarge pGEX-4T-1-3xHA-IκBα (1-62) Plasmid #129226 Purpose Encodes HA tagged human IκBα (1-62) for purification by GST affinity Depositor Kevin Janes Article WebTMEM192 Promoter ubc Tag / Fusion Protein HA (C terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site AgeI (not destroyed) 3′ cloning site …

WebJan 3, 2024 · We performed centrifugation at 20,000 × g to generate an organelle-enriched pellet, two-step sucrose density gradient centrifugation, enrichment by superparamagnetic iron oxide nanoparticles (SPIONs), and immunoprecipitation using a 3xHA tagged version of the lysosomal membrane protein TMEM192. WebOct 26, 2024 · Tmem192-3xHA and lysosomes were detected by immunofluorescence with antibodies to the HA epitope tag and the lysosomal marker LAMP2, respectively. Scale …

WebOct 26, 2024 · Tmem192-3xHA and lysosomes were detected by immunofluorescence with antibodies to the HA epitope tag and the lysosomal marker LAMP2, respectively. ... (r 2 = 0.95; Fig. 1G) and even with those obtained using the less preferable LAMP1-RFP-3xHA as the lysosomal antigen tag (r 2 = 0.95; Fig. S1C), mitigating concerns that expression of …

WebGSC#9 stably expressing the lysosomal protein TMEM192 tagged with either 3xHA (HA-lyso) or 2xFLAG (FLAG-lyso) were mechanically lysed. Lysosomes were immunoprecipitated with anti-HA magnetic beads. These immunopurified lysosome fractions were subsequently analyzed by western-blot and label-free quantification (LFQ) proteomic. rockwood fifth wheel 2893bsWebAug 13, 2024 · HA-TMEM192-isolated lysosomes represented a highly pure fraction as determined by the absence of markers for other cellular compartments such as … rockwood financialWeb> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M otterpawtradingWebMar 3, 2024 · In LysoIP, TMEM192–3xHA transfected cells are used to immunopurify lysosomal organelles on anti-HA beads, and, in parallel, TMEM192–2xFLAG transfected … rockwood fifth wheelsWebImmunohistochemical analysis of TMEM219 in immersion fixed paraffin-embedded sections of human prostate gland. Samples were incubated in TMEM219 polyclonal antibody ( … otter park clevelandWebDisclosed herein are methods and compositions for modulating MFSD12 expression and activity to treat diseases such as lysosomal storage diseases, including cystinosis. Also disclosed are methods of altering skin pigmentation and methods of screening for MFSD12 modulation agents. rockwood fifth wheel 2023WebLaflamme C, McKeever P, Kumar R, Schwartz J, Kolahdouzan M, Chen C, et al.Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. elife. 2024;8: pubmed publisher otter patch